Cell Rolling in Microfluidic Devices

Cell rolling is a process where cells injected at specific flow rates, thus subjected to shear stress, in a biofunctionalized microfluidic chamber interact with the biomolecules of the chamber through the formation of bonds related to specific adhesion molecules, thus resulting in rolling. The rolling of cells is a subject of particular interest in the scientific world. Microfluidic systems for cell rolling can be used for different applications like stem cell or circulating tumor cell, separation and isolation of cells and rare cells of particular interest, physiological studies on cells, cell interactions, and induction of apoptotic signals in cancer cells.


In this picture it is shown a microfluidic chamber in which microbeads are linked on the bottom surface and biofunctionalized with antibodies.

In our lab, we developed insitu microfluidic protocols which allow to biofunctionalize assembled microfluidic devices with antibodies. 

The biofunctionalised devices allow us to:
1) Study interaction between cells and biomolecules and observe phenotype changes;
2) Isolate cell of particulat diagnostic interest from an etherogenous population of cells.

REFERENCES:

1) Gerardo Perozziello, Giuseppina Simone, Natalia Malara, Rosanna La Rocca, Rossana Tallerico, Rossella Catalano, Francesca Pardeo, Patrizio Candeloro, Giovanni Cuda, Ennio, Carbone, Enzo Di Fabrizio "Microfluidic biofunctionalisation protocols to form multivalent interactions for cell rolling and phenotype modification investigations.” Accepted to Electrophoresis (2013)  
2) G. Simone, N. Malara, V. Trunzo, G. Perozziello, P. Neuzil, M. Francardi, L. Roveda, M. Renne, U. Prati, V. Mollace,  A. Manz,, E. Di Fabrizio,  “Protein–Carbohydrate Complex Reveals Circulating Metastatic Cells in a Microfluidic Assay”, Small (2013), DOI: 10.1002/smll.201202867  
3) G. Simone, G. Perozziello, E. Battista, F. De Angelis, P. Candeloro, F. Gentile, N. Malara, A. Manz, E. Carbone, P. Netti, E. Di Fabrizio. “Cell rolling and adhesion on surfaces in shear flow.
A model for an antibody-based microfluidic screening system”, Microelectronic Engineering, 2012, http://dx.doi.org/10.1016/j.mee.2012.07.008 
4) G. Perozziello, R. La Rocca, G. Cojoc, C. Liberale, N. Malara, G. Simone, P. Candeloro, A. Anichini, L. Tirinato, F. Gentile, M. Coluccio, E. Carbone and E.Di Fabrizio, “Microfluidic devices module tumor cell line susceptibility to NK cell recognition”, SMALL (2012), DOI: 10.1002/smll.201200160
5) G. Simone, P. Neuzil, G. Perozziello, N. Malara, M. Francardi, E. Di Fabrizio, A. Manz, “A Facile in situ microfluidic method to create multivalent surface: towards functional glycomics” . Lab on a chip (2012), ISSN: 1473-0197, DOI: 10.1039/C2LC21217J