Raman Spectroscopy

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In Raman spectroscopy, the sample is irradiated by intense laser beams in the UV-visible-IR region (n), and the scattered light is usually observed in the through the detector (CCD, photo-multiplier tube). The scattered light consists of two types: one, called Rayleigh scattering, is strong and has the same frequency as the incident beam (n), and the other, called Raman scattering, is very weak (~ 10-5 of the incident beam) and has frequencies n0nm, where nm is a vibrational frequency of a molecule.
The n0 - nm and n0 + nm lines are called the Stokes and anti-Stokes lines, respectively. Thus, in Raman spectroscopy, we measure the vibrational frequency nm) as a shift from the incident beam frequency n0). Raman measurements are straightforward, easy to perform, almost no sampling needed, whereas the analyses produce information on structures and spectral fingerprints for straightforward identifications.
In our laboratory BIONEM, we carry out measurements for the samples to be utilized as a surface enhanced Raman scattering (SERS) substrate. Various measurements are being performed on proteins, BRCT WT and mutated proteins, tumor cells, etc..  
 
a) The micro-Raman system (inVia Renishaw Raman microscope)
b) The sketch diagram of the Raman system.  

Characteristics

The micro-confocal Renishaw Raman system contains Ar+ ion Lasers with three different wavelength (457, 488, and 514 nm) and one diode laser with 830nm with possibilty to perform measurements in different polarizations to probe the orientation of molecules. Different gratings (1200, 1800, and 2400 lines/mm) in order to perform high resolution Raman spectrum Dn@ 0.9 cm-1).
Motorized XYZ sample stage with 108mm X 71mm with resolution of 0.1 um for xy. Mapping facility in different modes: Point and line mapping for 830 and 514 nm, to map the interested region at any wave number with auto-focussing option even during the measurements. To perform the macro-Raman measurements for the bulk sample. To perform the Raman measurements for the sample located at distance by means of using Fiber-optic probe. Sample chamber temperature can be varied from liquid-N2 temp. to up to 600°C. Objectives (5X, 20X, 50X, 100X, 150X and 50X-long working distance) and possibility to scan z-axis using piezo for objective.

Manuals and link

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